After callus formation, culturing on a low auxin or hormone free media will promote somatic embryo growth and root formation.
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It also offers to confirm single cell origin of somatic embryos and the asymmetry of the first cell division, which starts the process.
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In fact, there has been shown to be a correlation between mitochondrial activities and programmed cell death ( PCD ) during somatic embryo development.
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Plant cells can also be induced to form embryos in plant tissue culture; such embryos are called " somatic embryos " and can be used to generate new plants from single cells.
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After the PEMBSIS cell culture chambers were recovered from the Shuttle, specimens of living cells and somatic embryos were photographed, counted, and chemically fixed within nine hours of landing, before their first division cycle on Earth was complete.
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Although many simpler proteins can be produced using rDNA in bacterial cultures, more complex proteins that are somatic embryos as a source for direct gene transfer via particle bombardment, transit gene expression and confocal microscopy observation is one of its applications.
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